Long-read de novo assemblies: Glossary

Key Points

Introduction	This part of the lesson will mostly be given in the form of a lecture
Setup for the workshop	VM can be used after the workshop Installing required packages on own machine may require root privileges.
QC and evaluation of long read data	PacBio and Nanopore have read lengths of many kilobases In practice quality of input DNA, library prep. and many other factors will determine read length distribution PacBio and Nanopore have different types of errors
Assembly of long reads (and Illumina reads)	Each platform and each data sets requires hands-on work with the assembler
Comparison and visualization of long read assemblies	(Near-) exact contigs show up as a diagonal line in a mummerplot Repeat content shows up as dots / lines across the image
Validation of assemblies
Summary of obtained results and closing

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